- Bushrod Washington family papers, 1662/18353
- Early Records of the Mount Vernon Ladies' Association, 1853/1951, bulk 1853/1875266
- Historic ephemera collection, 1796/19863
- Historic manuscript collection, 1601/193310
- Historical Photograph Collection, 1850/20104
- John Augustine Washington III and Mount Vernon Ladies' Association (MVLA) collection, 1834/19571
- Lily Laughton Papers, 1850/18914
- Mount Vernon Black and White Film Collection, 1933/20054
- Papers of the Superintendent and Resident Director, 1850/19963
- Publications - History of Mount Vernon and George Washington, 1854/20123
- Publications and Printed Material of the Mount Vernon Ladies' Association, 1854/20163