- Chapin collection of Washington & Custis family documents, 1758/18291
- Esther Maria Lewis Chapin collection of Washington family documents, 1743/18521
- George Washington collection, 1654/17992
- Historic ephemera collection, 1796/19863
- Historic manuscript collection, 1601/19332
- Historical Photograph Collection, 1850/20102
- Martha Washington collection, 1761/18023
- Mount Vernon Black and White Film Collection, 1933/20051
- Peter family papers, 1722/19325
- Robert Waldsmith Collection of stereographs and cabinet cards, 1856/19811
- Sarah Tracy Collection, 1859/18681