- Audio Visual Collection, 1928/20156
- Financial Records of the Mount Vernon Ladies' Association, 1858/20153
- Geo. Gregory Smart collection of Washington and Mount Vernon ephemera, 1793/20173
- George Washington's Mount Vernon Magazine, 20153
- Guest Books and Visitor Registers, 1850/20161
- Historical Photograph Collection, 1850/20101
- Justin Glenn collection of Washington family genealogy, 2013/20181
- Licensing Department Files, 1984/20141
- Measured Drawings and Blueprints of Mount Vernon, 1870/20161
- Mount Vernon and MVLA Ephemera, 1858/20221
- Oral Histories of the Mount Vernon Ladies' Association, 1957/20152
- Papers of the Mount Vernon Ladies' Association, 1858/201640
- Publications - History of Mount Vernon and George Washington, 1854/20122
- Publications and Printed Material of the Mount Vernon Ladies' Association, 1854/201611
- Superintendent's Letter Books, Diaries, and Monthly Reports - collection of bound volumes, 1885/19672