- Papers of the Superintendent and Resident Director, 1850/1996195
- Restoration Files for the Historic Structures Report, 1750/2005163
- Papers of the Mount Vernon Ladies' Association, 1858/2016134
- Mount Vernon Black and White Film Collection, 1933/2005126
- Financial Records of the Mount Vernon Ladies' Association, 1858/201550
- Papers of Christine Meadows, Curator, 1960/199731
- Operation Overview Collection, 1953/198315
- Publications and Printed Material of the Mount Vernon Ladies' Association, 1854/201615
- Geo. Gregory Smart collection of Washington and Mount Vernon ephemera, 1793/201714
- Historic ephemera collection, 1796/19869
- Audio Visual Collection, 1928/20158
- Publications Committee Collection, 1951/19978
- John Augustine Washington III and family papers, 1789/19947
- Historical Photograph Collection, 1850/20106
- George Washington Memorial Parkway History Collection, 1900/20015