- Early Records of the Mount Vernon Ladies' Association, 1853/1951, bulk 1853/18752
- Financial Records of the Mount Vernon Ladies' Association, 1858/20151
- Harrison H. Dodge Papers, 1816/19361
- Historic manuscript collection, 1601/19332
- Historical Photograph Collection, 1850/201016
- John Augustine Washington III and family papers, 1789/19943
- Maps of Mount Vernon and the surrounding areas, 1857/19921
- Measured Drawings and Blueprints of Mount Vernon, 1870/20161
- Mount Vernon Black and White Film Collection, 1933/20055
- Papers of the Mount Vernon Ladies' Association, 1858/20167
- Papers of the Superintendent and Resident Director, 1850/19966
- Publications and Printed Material of the Mount Vernon Ladies' Association, 1854/20162
- Superintendent's Letter Books, Diaries, and Monthly Reports - collection of bound volumes, 1885/19671