- Historical Photograph Collection, 1850/2010198
- Restoration Files for the Historic Structures Report, 1750/2005133
- Peter family papers, 1722/193268
- Robert Waldsmith Collection of stereographs and cabinet cards, 1856/198154
- Papers of the Mount Vernon Ladies' Association, 1858/201645
- Papers of the Superintendent and Resident Director, 1850/199632
- Geo. Gregory Smart collection of Washington and Mount Vernon ephemera, 1793/201723
- Superintendent's Letter Books, Diaries, and Monthly Reports - collection of bound volumes, 1885/196718
- Early Records of the Mount Vernon Ladies' Association, 1853/1951, bulk 1853/187516
- Financial Records of the Mount Vernon Ladies' Association, 1858/201515
- Historic newspapers collection, 1757/195711
- Publications and Printed Material of the Mount Vernon Ladies' Association, 1854/201610
- John Augustine Washington III and family papers, 1789/19948
- Morley Jeffers Williams Collection, 1602/19678
- Harrison H. Dodge Papers, 1816/19367