- Austin-Twyman Papers, 1765/193931
- James Washington Singleton Papers, 1770/1975, bulk 1850/192012
- Geo. Gregory Smart collection of Washington and Mount Vernon ephemera, 1793/201711
- J. Hoge Tyler Family Collection, 1802/195611
- Early Records of the Mount Vernon Ladies' Association, 1853/1951, bulk 1853/187510
- John L. Nau III Civil War History Collection, 1806/1988, bulk 1861/18658
- Manuscripts - Geographic Names, Business Names and Subjects, 1725/19498
- Robert Morton Hughes Papers, 1754/19508
- Ancella Bickley, Historian, Research Papers regarding African-Americans, 1775/2018, bulk 1970/20117
- Brown, Coalter, Tucker Papers (II), 1791/19207
- Cazenove Family Papers (MS212), 1786/19707
- Dickson Family Papers, 1769/19247
- Wharton and Radford Families Papers, 1783/19067
- Blow Family Papers, 1613/1960, bulk 1770/18756
- Emory L. Kemp Papers regarding Industrial History, 1735/20216