- John N. Paden papers, 1938/20102
- John Smith Mezzotint Collection, 1677/17521
- John W. Warner papers, 1967/2009, bulk 1967/20081
- John Womack Wright Collection of Maps, 1675/19183
- Johnston Family Genealogy, 1740/18501
- Joseph C. Cabell and the Cabell Family papers
1731-19171
- Latane Family Papers
1650-18989
- Latin American Map Collection, 1711/18731
- Lee Nicholson Papers, 1827/19385
- Lexington Arsenal and Virginia Militia records, 1702/18393
- Livre pour la remede pour les chevaux [Book of the remedy for horses], 1741/17601
- Lonnie L. Okes, Compiler, Akers, Lilly, and Ellison Families History, 1700/19611
- Lyman C. Draper, Antiquarian, Manuscripts, 1740/18151
- Lynch Family History, 1600/19401
- Maben Jones Papers2