- Alleghany Chapter, National Society of the Daughters of the American Revolution Records, 1890/20221
- Carter/Cash Family Collection, 1940/20091
- Elizabeth Fine Papers, 1983/20121
- Hard Times Blues Collection, 1966/20191
- J. Hoge Tyler Diary, 1846/2009, bulk 1846/19251
- John M. and Cora Bolton McBryde Family Papers, 1862/20181
- Lucy Herndon Crockett Papers, 1940/20151
- Margaret M. Sullivan Collection, 1932/20111
- Milka T. Bliznakov Papers and Architectural Drawings, 1946/20101
- New River Community Action, Inc., Records, 1966/20151
- Peacock-Harper Culinary History Friends Records, 1999/20191
- Records of the American Association of University Women (AAUW), Virginia Tech Chapter, 1926/20081
- Rick Boucher Papers, 1968/2017, bulk 1975/20111
- Roanoke Appalachian Trail Club Records, 20251
- Roanoke Valley Academy of Medicine Records, 1874/20161